Journal: Frontiers in Molecular Biosciences
Article Title: Proximity-dependent biotinylation technologies for mapping RNA-protein interactions in live cells
doi: 10.3389/fmolb.2022.1062448
Figure Lengend Snippet: Summary of strengths, limitations and available tools of the current PDB strategies.
Article Snippet: AVAILABLE TOOLS , • Plasmid to clone BoxB‐tagged RNAs (Addgene #107253) , , • Plasmid encoding for MCP-APEX2 (Addgene #154936) , , • Plasmid encoding for CARPID BASU-dCasRx (Addgene #153209), CARPID dCasRx-BASU (Addgene #153303), dCas13d-dsRBD-APEX2 (Addgene #154939) , • Addgene plasmids encoding for APEX2-OMM (#79056), ERM-APEX2 (#79055), mito-APEX2 (#72480), APEX2-SENP (#129276), APEX2-eIF41 (#129645), APEX2-eIFE1 (#129644), C1-APEX2 (#129641), APEX2-FBL (#187577), APEX2-SRSF7 (#187582), APEX2-SRSF1 (#187575), APEX2-PML (#187583), APEX2-SP100 (#187584), APEX2-NPAT (#187585), APEX2-LMNA (#187576).
Techniques: CRISPR, Purification, Binding Assay, Plasmid Preparation, Expressing, Positive Control